Protection of Women and Girls in Sports Act of 2025

This bill amends federal education law to prohibit federally funded athletic programs from allowing individuals assigned male at birth to participate in sports designated for women or girls. It defines "sex" for this purpose as biological sex at birth, based on reproductive anatomy and genetics. The law directly affects schools, colleges, and sports organizations receiving federal funding. Violations would constitute a breach of Title IX, requiring programs to exclude individuals whose sex is male from women's or girls' athletic teams.