Keep Our Girls Safe Act of 2025

This bill amends Title IX to prohibit individuals assigned male at birth (based on reproductive biology and genetics at birth) from using locker rooms designated for females during active use in school programs. It directly affects transgender girls and schools implementing gender-segregated facilities. The key provision makes it unlawful under federal law to use such facilities when they are actively used by individuals of a different sex. The policy change takes effect 30 days after enactment.